HOW DEEP IS DEEP ENOUGH FOR RNA-SEQ PROFILING OF BACTERIAL TRANSCRIPTOMES?

Abstract Background High-throughput sequencing of cDNA libraries (RNA-Seq) has proven to be a highly effective approach for studying bacterial transcriptomes.A central challenge in designing RNA-Seq-based experiments is estimating a priori the number of reads per sample needed to detect and quantify thousands of individual transcripts with a large

In Germany, the fall of the Iron Curtain led to the extensive withdrawal of allied troops stationed there, as well as the reduction in number of the German armed forces.This process was accompanied by the repurposing of formerly restricted Carpet Shampooers military terrain in both urban contexts and the countryside.Post-military landscapes are ful